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monocytic cell line u937  (ATCC)


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    ATCC monocytic cell line u937
    Monocytic Cell Line U937, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6925 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monocytic+cell+lines+u937/U-937/pm41786067-80-13-17
    Average 99 stars, based on 6925 article reviews
    monocytic cell line u937 - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: ANGPTL8 links refeeding to monocyte dynamics and metabolic inflammation via the CCL5-CCR5 axis
    Article Snippet: The human monocytic cell lines U937 and THP-1 were obtained from the ATCC.

    Article Title: Infection and nuclear interaction in mammalian cells by ‘ Candidatus Berkiella cookevillensis’, a novel bacterium isolated from amoebae
    Article Snippet: Confocal microscopy was performed using a Zeiss AxioObserver microscope with LSM700 confocal module and Plan-Apochromat 63x/1.40 Oil DIC M27 objective.

    Cell Culture:

    Article Title: Leishmania Infection Induces MicroRNA hsa-miR-346 in Human Cell Line-Derived Macrophages
    Article Snippet: .. The human monocytic cell lines U937 (ATCC CRL-1593.2) and THP-1 (ECACC 88081201) were routinely cultured in RPMI-1640 medium supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS), 2 mM L-glutamine, 1% penicillin/streptomycin at 37°C and 5% CO 2 . ..

    Article Title: Leishmania Infection Induces MicroRNA hsa-miR-346 in Human Cell Line-Derived Macrophages.
    Article Snippet: .. The human monocytic cell lines U937 (ATCC CRL-1593.2) and THP-1 (ECACC 88081201) were routinely cultured in RPMI-1640 medium supplemented with 10% heatinactivated Fetal Bovine Serum (FBS), 2 mM L-glutamine, 1% penicillin/streptomycin at 37◦C and 5% CO2. ..

    Article Title: Neisseria gonorrhoeae survives within and modulates apoptosis and inflammatory cytokine production of human macrophages
    Article Snippet: .. Cell culture and differentiation The human monocytic cell lines U937 and THP-1 were obtained from ATCC and maintained at 2.5 × 10 5 in RPMI 1640 medium supplemented with 10% FBS at 37°C and 5% CO 2 . .. U937 and THP-1 cells at 1.10 6 cells/ml were differentiated to macrophages using 80 nM phorbol 1-myristate 13-acetate (PMA, Sigma-Aldrich) for 2 days in 24 well (1ml/well) or 48 well (0.5ml/well) tissue culture plates, and when necessary on coverslips for microscopy.

    Article Title: Leishmania infantum Induces Mild Unfolded Protein Response in Infected Macrophages.
    Article Snippet: .. Cell culture, treatment and infection The human monocytic cell lines U937 (ATCC CRL-1593.2) and THP-1 (ATCC TIB-202) were cultured in a humidified incubator at 37 ̊C and 5% CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS), 2 mM L-glutamine, 10 g/l Non-Essential Amino Acid, 100 μg/ml streptomycin, 100 U/l penicillin (complete medium). .. To induce differentiation into macrophages-like cells, 6 x 105 cells were seeded in 35 mm dishes and treated with 10 ng/ml phorbol myristic acid (PMA) for 24 h. Then, the medium was replaced with regular complete medium and cells were incubated for a further 48 h. Murine primary macrophages were removed from seven ICR/CD-1 mice (Harlan Nossan, Milan, Italy) by peritoneal washing and cultured in RPMI-1640 complete medium overnight at 37 ̊C in a humidified 5% CO2 atmosphere.

    Infection:

    Article Title: Leishmania infantum Induces Mild Unfolded Protein Response in Infected Macrophages.
    Article Snippet: .. Cell culture, treatment and infection The human monocytic cell lines U937 (ATCC CRL-1593.2) and THP-1 (ATCC TIB-202) were cultured in a humidified incubator at 37 ̊C and 5% CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS), 2 mM L-glutamine, 10 g/l Non-Essential Amino Acid, 100 μg/ml streptomycin, 100 U/l penicillin (complete medium). .. To induce differentiation into macrophages-like cells, 6 x 105 cells were seeded in 35 mm dishes and treated with 10 ng/ml phorbol myristic acid (PMA) for 24 h. Then, the medium was replaced with regular complete medium and cells were incubated for a further 48 h. Murine primary macrophages were removed from seven ICR/CD-1 mice (Harlan Nossan, Milan, Italy) by peritoneal washing and cultured in RPMI-1640 complete medium overnight at 37 ̊C in a humidified 5% CO2 atmosphere.



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    PCA,principal component analysis of differentially expressed genes in PRF-activated <t>U937</t> and THP-1 macrophages. The plot depicts the sample distribution in a two-dimensional space defined by the first and second principal components of the covariance matrix. Gene expression values were normalized and expressed as logCPM, log counts per million.
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    PCA,principal component analysis of differentially expressed genes in PRF-activated U937 and THP-1 macrophages. The plot depicts the sample distribution in a two-dimensional space defined by the first and second principal components of the covariance matrix. Gene expression values were normalized and expressed as logCPM, log counts per million.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: PCA,principal component analysis of differentially expressed genes in PRF-activated U937 and THP-1 macrophages. The plot depicts the sample distribution in a two-dimensional space defined by the first and second principal components of the covariance matrix. Gene expression values were normalized and expressed as logCPM, log counts per million.

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Gene Expression

    Volcano plot analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF. Volcano plots illustrate upregulated (red) and downregulated (blue) genes in U937 and THP-1 cells following PRF exposure. The annotated data points represent the 50 genes with the greatest Euclidean (Manhattan) distance from the origin that exceed the defined significance thresholds (dashed lines). Thresholds were set at –log 10 (p) ≥ 2.0 and |log 2 fold change| ≥ 3.0.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: Volcano plot analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF. Volcano plots illustrate upregulated (red) and downregulated (blue) genes in U937 and THP-1 cells following PRF exposure. The annotated data points represent the 50 genes with the greatest Euclidean (Manhattan) distance from the origin that exceed the defined significance thresholds (dashed lines). Thresholds were set at –log 10 (p) ≥ 2.0 and |log 2 fold change| ≥ 3.0.

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques:

    Heatmap of differentially expressed genes in U937 and THP-1 macrophages exposed to PRF lysates. The heatmap visualizes the 395 upregulated and 372 downregulated genes in U937 cells, and the 116 upregulated and 149 downregulated genes in THP-1 cells, following exposure to PRF lysates. Differentially expressed genes were defined by an adjusted p-value < 0.05. The dataset integrates transcriptional data from three independent PRF preparations. Color gradients indicate relative expression levels, with red representing upregulation and blue representing downregulation; color intensity reflects the magnitude of the expression change.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: Heatmap of differentially expressed genes in U937 and THP-1 macrophages exposed to PRF lysates. The heatmap visualizes the 395 upregulated and 372 downregulated genes in U937 cells, and the 116 upregulated and 149 downregulated genes in THP-1 cells, following exposure to PRF lysates. Differentially expressed genes were defined by an adjusted p-value < 0.05. The dataset integrates transcriptional data from three independent PRF preparations. Color gradients indicate relative expression levels, with red representing upregulation and blue representing downregulation; color intensity reflects the magnitude of the expression change.

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing

    Venn analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF lysates. Venn diagrams show the overlap of upregulated and downregulated genes between U937 and THP-1 cells after exposure to PRF lysates (adjusted p < 0.05). A total of 28 genes were commonly upregulated, and 20 genes were commonly downregulated in both cell lines.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: Venn analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF lysates. Venn diagrams show the overlap of upregulated and downregulated genes between U937 and THP-1 cells after exposure to PRF lysates (adjusted p < 0.05). A total of 28 genes were commonly upregulated, and 20 genes were commonly downregulated in both cell lines.

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques:

    Functional enrichment analysis of upregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis, also known as over-representation analysis (ORA), was performed using the g:Profiler web-based platform. The plot depicts the top significantly enriched pathways derived from Gene Ontology and other integrated databases. Pathways are ranked and labeled numerically according to significance. P-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: Functional enrichment analysis of upregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis, also known as over-representation analysis (ORA), was performed using the g:Profiler web-based platform. The plot depicts the top significantly enriched pathways derived from Gene Ontology and other integrated databases. Pathways are ranked and labeled numerically according to significance. P-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Functional Assay, Derivative Assay, Labeling

    Functional enrichment analysis of downregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis (over-representation analysis, ORA) was performed using the g:Profiler web-based platform. The figure displays the top significantly enriched pathways identified from Gene Ontology and other integrated databases, ranked and labeled numerically by significance. p-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: Functional enrichment analysis of downregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis (over-representation analysis, ORA) was performed using the g:Profiler web-based platform. The figure displays the top significantly enriched pathways identified from Gene Ontology and other integrated databases, ranked and labeled numerically by significance. p-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Functional Assay, Labeling

    RT–qPCR validation of selected genes in U937 macrophages stimulated with PRF lysates. U937 macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. Data represent the median of four independent experiments and two gene panels (A, B) .

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: RT–qPCR validation of selected genes in U937 macrophages stimulated with PRF lysates. U937 macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. Data represent the median of four independent experiments and two gene panels (A, B) .

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Biomarker Discovery

    RT–qPCR and ELISA analysis of inflammatory gene expression in U937 and THP-1 macrophages stimulated with PRF lysates. U937 (A, B) and THP-1 (C, D) macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. ELISA quantified CXCL8 protein levels in the corresponding cell culture supernatants. Data represent the median of independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

    doi: 10.3389/fimmu.2026.1722342

    Figure Lengend Snippet: RT–qPCR and ELISA analysis of inflammatory gene expression in U937 and THP-1 macrophages stimulated with PRF lysates. U937 (A, B) and THP-1 (C, D) macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. ELISA quantified CXCL8 protein levels in the corresponding cell culture supernatants. Data represent the median of independent experiments.

    Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Gene Expression, Cell Culture